What is a Secondary Antibody and how does it work?
A secondary antibody binds with a primary antibody that is directly attached to the target antigen. Exploiting this interaction allows secondary antibodies to indirectly help in the detection and purification of target proteins (antigens). Secondary antibodies are staple tools for detecting, quantifying, or purifying target proteins and other molecules. Ideal secondary antibody should bind specifically to the primary antibody, which is determined largely by the latter’s host species and antibody class or isotype (IgG, IgA, IgD, IgE, and IgM). Secondary antibodies are conjugated to another molecule for detection, such as to a fluorophore, biotin, horseradish peroxidase, or alkaline phosphatase , thereby making them more sensitive and flexible for use in labeling and detection. Thus, they are used in the colorimetric, chemiluminescent, and fluorescent detection of primary antibodies in a series of applications including flow cytometry, western blotting, and cell imaging. Pro Lab offers top quality secondary antibodies at competitive rates.