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Oligo
Synthesis
-from
GenScript Corporation
High
purity (>95%) : GenScript use a novel and proprietary method:
GAP (GenScript Affinity Purification via Affinity Column), which
provides high purity (>95%), which is comparable to PAGE and HPLC
methods, and is much higher than the purity of regular De-salted
primers (30% - 60%), OPEC and cartridge purified primers. GAP
primers are suitable for many downstream experiments, including
PCR, DNA sequencing, and gene chips.
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Modifications
Available
Phosphorothioated
"U" or "I"
3' or 5' Biotin 3
' or 5' Amine(3 is C7, 5 is C6)
3' or 5' Fluorescence FAM, or TET, or Hex
3' or 5' TAMRA
5' Fam - 3' Dabcyl
3' or 5' DIG
3' or 5' -SH or HS-SH
5' -CY3 or CY5 5' Fam, or TET, or Hex - 3' Tamra (Real-Time
PCR Probe)
5' or 3' PHOSPHATE
5' Fam - 3' BHQ-1
5' ROX - 3' BHQ-2
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Turn-around time:
- For GAP purification: 7-8 business days
- For PAGE and HPLC modified oligos: 8-10 business days.
- Modified and long oligos: extra 1-2 business days.
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Custom Gene Synthesis
- from GenScript Corporation
- USA
Synthesis
of gene between 50 bp to 10 kb, with guaranteed 100% match with
your requested sequence. Full confidentiality to your sequence.
Several reputed satisfied clients from Govt. Institutes & Research
and Development Departments of Pharma Companies. Unbelievable
competitive price.
Email
or call
us for technical
& commercial discussions.
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PCR
Cloning, Subcloning and Mutagenesis Service
- from GenScript Corporation - USA
Deliver about 5 ug of plasmid with the sequence information to customer.
Email
or call
us for technical
& commercial discussions.
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DNA
Vector based siRNA
- from GenScript Corporation - USA
This
is a comprehensive package including siRNA design, siRNA vector,
and custom construction. Using this technology, GenScript can
make siRNA construct easily for you with a minimal cost. GenScript
siRNA vectors carry cGFP (coral GFP) to track transfection efficiency,
include inducible vectors and multiple viral expression systems.
GenScript will synthesize the DNA insert, clone the insert into
the vector that you desired, and sequencing verify it. The final
plasmid (Miniprep or Maxiprep) will then be delivered to you together
with the sequence data and purified product. Email
or call
us for technical & commercial discussions.
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Protein Expression and Purification
- from GenScript Corporation - USA
GenScript
is a world leader in protein expression and purification service.
GenScript's service is a complete solution for your needs, and
helps you to overcome your cloning and protein expression bottleneck.
Genscript offers a variety of protein expression systems including
bacteria, baculovirus/insect, and yeast from small scale to large
scale with a full-range of purification options. The services
include: gene synthesis, generating expression construct, establishing
stable cell line, generating recombinant virus, small-scale expression
and purification, large scale expression and purification.
Email
or call
us for technical & commercial discussions.
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Polyclonal
Antibody Production Service
- from GenScript Corporation - USA
GenScript is a world leader in custom antibody services. Many
members of our technical staffs have more than 10 years' experience
producing high-quality antibodies for the life science research
community
Comprehensive service package:
Antigen design - Online Antigen Design Tool (available on Genscript
website)
Peptide synthesis
Polyclonal antibody
Email
or call
us for technical
& commercial discussions.
| Cat. No. |
Service
Items |
| SC1030 |
Partial
polyclonal antibody production (Rabbit) |
| SC1015 |
Complete
peptide polyclonal antibody package (Rabbit) |
| SC1031 |
Complete
affinity-purified polyclonal antibody package (Rabbit) |
| SC1044 |
Phospho-specific
polyclonal antibody production (rabbit) |
| SC1045 |
Complete
protein A purified peptide polyclonal antibody package (Rabbit) |
| SC1046 |
Complete
polyclonal peptide antibody package (Mouse) |
| SC1047 |
Partial
polyclonal peptide antibody package (Mouse) |
| SC1048 |
Complete
affinity-purified polyclonal antibody package(Mouse) |
| SC1049 |
Complete
protein A purified peptide polyclonal antibody package (Mouse) |
| SC1050 |
Complete
peptide polyclonal antibody package (Goat) |
| SC1051 |
Complete
protein A purified peptide polyclonal antibody package (Goat) |
| SC1052 |
Partial
polyclonal antibody production package (Goat) |
| SC1032 |
Peptide
antigen design |
| SC1033 |
Additional
rabbit |
| SC1034 |
Extended
protocol |
| SC1020 |
Peptide
synthesis |
| SC1035 |
KLH conjugation
|
| SC1036 |
Titer test
by ELISA |
| SC1037 |
Western
blot testing |
| SC1038 |
Antibody
purification by protein A |
| SC1039 |
Antibody
purification by peptide affinity column |
Monoclonal
Antibody Production Service
- from GenScript Corporation - USA
GenScript is a world leader in custom antibody services.
Many members of our technical staffs have more than 10 years'
experience producing high-quality antibodies for the life science
research community
Comprehensive service package:
Antigen design - Online Antigen Design Tool available on Genscript
website
Peptide synthesis
Monoclonal antibody
Email
or call
us for technical & commercial discussions.
| Cat#
|
Service
Items |
| SC1040 |
Monoclonal
antibody without peptide synthesis |
| SC1041 |
Monoclonal
antibody with peptide synthesis (15 aa including cysteine,
90% purity, 20 mg) |
| SC1032 |
Peptide
antigen design |
| SC1020 |
Custom
peptide synthesis |
| SC1042 |
Ascites
production (5 mice) |
| SC1043 |
Protein
A or G purification for 10 ml ascites |
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Western
Blotting Services
- from Zyagen - USA
Zyagen offers Western blotting services to make a ready-to-use
Western blots of the client specifications using total protein
(tissue lysates) extracted from rat and mouse tissues available
at Zyagen. for list of tissues available with zyagen, please
contact pro lab. The custom service scientists can also process
total protein or tissues for extraction of protein provided
by the investigators for construction of Western blots of their
choice. To ensure the highest quality blots, each protein sample
whether provide by the client or Zyagen is subjected to rigorous
quality control procedures before the electrophoresis. Zyagen
can make blots with up to 15 tissues each in addition to the
protein marker.
| Cat# |
Title
of the services |
Description |
| SWB-100 |
Extraction
of tissue lysates (total protein) from rat or mouse tissues
available at Zyagen or tissues provided by the client.
For the list of available tissues from Zyagen, please
contact Pro Lab. |
Service
includes extraction of tissue lysates and quantification
of total protein by a sensitive accurate assay, and verification
of purity and integrity of the extracted protein. |
| SWB-200 |
Complete
Western blotting services using rat or mouse tissues available
at Zyagen. For the list of available tissues from Zyagen,
please contact Pro Lab. |
Service
includes extraction of total protein, fractionation on
SDS-PAGE, and transfer to PVDF membranes. Choose up to
15 samples per each membrane. |
| SWB-300 |
Complete
Western blotting services using tissues provided by the
client |
Service
includes extraction of total protein from the provided
tissues , fractionation on SDS-PAGE, and transfer to PVDF
membranes. Choose up to 15 samples per each membrane.
|
Email
or call
us for technical & commercial discussions.
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Northern
Blotting Custom Services
- from Zyagen - USA
Zyagen offers Northern blotting services to extract
total RNA, purification of mRNA and construct ready-to-hybridize
Northern blots of the client specifications using RNA extracted
from rat and mouse tissues available at Z
| Service
I.D. |
Title of the services |
Description |
| SNB-100 |
Isolation
of total RNA from rat or mouse tissues available at Zyagen
or tissues provided by the client. Ask us for the tissue
available at Zyagen. |
Service
includes extraction of RNA, treatment with RNase-free
DNase, quantification by sensitive assay, and verification
of purity and integrity of the isolated RNA.
|
| SNB-200 |
Isolation
of poly (A+) RNA from rat or mouse tissues available at
Zyagen or tissues provided by the client. Ask us for the
tissue available at Zyagen. |
Service
includes extraction and purification of poly (A+) RNA,
treatment with RNase-free DNase, quantification by sensitive
assay, and verification of purity and integrity of the
purified mRNA.
|
| SNB-300 |
Complete
Northern blotting services using total RNA isolated from
rat or mouse tissues available at Zyagen. Ask us for the
tissue available at Zyagen.
|
Service
includes isolation of total RNA, fractionation on denatured
agarose gel (20mg/sample/lane), and transfer to nylon
or nitrocellulose membranes. Choose up to 15 tissues per
each membrane.
|
| SNB-400 |
Complete
Northern blotting services using mRNA isolated from rat
or mouse tissues available at Zyagen. Ask us for the tissue
available at Zyagen.
|
Service
includes isolation of poly (A+) RNA, fractionation on
denatured agarose gel (2mg/sample/lane) , and transfer
to nylon or nitrocellulose membranes. Choose up to 15
samples per each membrane.
|
| SNB-500 |
Complete
Northern blotting service using total RNA, mRNA, or tissues
provided by the investigator |
Service
includes isolation of total RNA or poly (A+) RNA from
the provided tissues, fractionation on denatured agarose
gel, and transfer to nylon or nitrocellulose membranes.
Choose up to 15 samples per each membrane.
|
yagen. To ensure the highest quality blots, each
RNA sample whether provide by the client or Zyagen is subjected
to rigorous quality control procedures before the electrophoresis.
|
Email
or call
us for technical & commercial discussions.
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Immunohistochemistry
Services
- from Zyagen - USA
Zyagen offers immunohistochemistry services for pharmaceutical,
biotechnology, and academic research institutes using different
method of detection (fluorescent, alkaline phosphatase, and peroxidase)
on paraffin and frozen tissue sections.
Complete immunohistochemistry (IHC) custom services include:
Affinity purification of antibody: Zyagen offers Protein A, Protein
G, and specific antigen affinity purification of the primary antibodies.
Optimization of tissue fixation process: This includes selection
of suitable fixative agent and determine the optimal time of fixation.
Optimization of antigen retrieval conditions: We are using detergent,
enzymatic, or microwave retrieval methods to expose the target antigen
to the antibody.
Titration of the primary antibody concentration and adjusting the
optimal experimental conditions of immunostaining assay.
Interpretation of immunostaining results by our experts scientists.
Image analysis and quantification of immunostaining if requested
by the investigator. Providing investigators with a report of the
findings with the microscopic images by e-mail. Color prints are
available with an additional charge. Email
or call
us for technical & commercial discussions.
| Cat# |
Title
of Service |
Description
|
| SIH-100 |
Antibody
Affinity purification |
Protein
A, protein G, or specific antigen affinity purification of polyclonal
antibodies. Investigator only provides the antibody. For the
antigen affinity purification, investigator may also provide
peptide used in the immunization or just the amino acid sequence. |
| SIH-200 |
Complete
IHC using Zyagen tissue sections |
Immunolocalization
of the antigen of interest in any rat or mouse tissue using
Zyagen tissue sections. Ask us for rat and mouse tissues available
from zyagen. Investigator only provides the primary antibody.
Zyagen may provide the antibody on the client cost.
|
| SIH-300 |
Complete
IHC using the investigator tissues |
Immunolocalization
of the antigen of interest in any human, rat, mouse, or other
species tissues. Investigator provides the primary antibody
and tissue sections or tissue blocks. Zyagen may provide the
antibody on the client cost. |
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Tissue Sectioning Custom Services
- from Zyagen - USA
Zyagen offers services for preparation of tissue sections and tissue
microarrays in the client specifications using tissues available at
Zyagen.
Zyagen routinely prepare tissue sections and tissue microarrays in
RNase-, DNase-, and protease-free conditions and are suitable for
in situ hybridization, in situ PCR, and immunohistochemistry.
Preparation of paraffin and frozen sections of any tissue of any rat
and mouse strain (up to 4 sections of each tissue per slide).
Preparation of paraffin and frozen tissue macroarrays (up to 15 different
tissues per slide).
Preparation of tissue microarrays (up to 60 tissues per slide).
Preparation of brain and spinal cord paraffin and frozen sections
(thin and thick floating sections).
Preparation of whole embryo paraffin and frozen sections.
Email
or call
us for technical & commercial discussions.
| Cat#
|
Title
of Services |
Description |
| STS-100 |
Tissue
sections |
Frozen
or paraffin sections for any rat or mouse tissue per slide.
Investigator chooses tissue type, procedures of tissue preparation
including fixation, thickness of tissue sections, and quantity
of slides. Ask us for tissues
available from Zyagen. |
| STS-200 |
Tissue
microarrays |
Paraffin
sections of rat or mouse tissues up to 60 tissues (one section
each) or up to 30 tissues (2 sections each) on one slide. Each
section is of 2 mm in diameter. Investigator chooses tissues
type, procedures of tissue preparation including fixation, thickness
of tissue sections, number of tissues per slide, and quantity
of slides. Ask us for tissues
available from Zyagen. |
| STS-150 |
Tissue
macroarrays |
Paraffin
or frozen sections for multiple rat or mouse tissues up to 15
per slide. Investigator chooses tissues type, number of tissues
per slide, procedures of tissue preparation, including fixation,
thickness of tissue sections, and quantity of slides.Ask
us for tissues from Zyagen. |
| STS-250 |
Coronal
brain sections* |
Paraffin
or frozen brain sections of 5-10 microns thickness in coronal
orientation for any region or specific area of adult rat or
mouse brain. Investigator chooses brain region/area, procedures
of tissue preparation including fixation, thickness of tissue
sections, and quantity of slides. |
| STS-300 |
Sagittal
brain sections* |
Paraffin
or frozen brain sections of 5-10 microns thickness in sagittal
orientation for any region or specific area of adult rat or
mouse brain. Investigator chooses brain region/area, procedures
of tissue preparation including fixation, thickness of tissue
sections, and quantity of slides. |
| STS-350 |
Horizontal
brain sections* |
Paraffin
or frozen brain sections of 5-10 microns thickness in horizontal
orientation for any region or specific area of adult rat or
mouse brain. Investigator chooses brain region/area, procedures
of tissue preparation including fixation, thickness of tissue
sections, and quantity of slides. |
| STS-400 |
Floating
coronal, sagittal, or horizontal brain sections* |
Frozen
sections of 20-60 microns thickness for any region or specific
area of adult rat or mouse brain in coronal, sagittal, or horizontal
orientation. Investigator chooses brain region/area, procedures
of tissue preparation including fixation, thickness of tissue
sections, and quantity of floating sections. |
| STS-450 |
Coronal
spinal cord sections |
Paraffin
or frozen spinal cord sections of 5-10 microns thickness in
coronal orientation for cervical, thoracic, lumber, or sacral
regions of adult rat or mouse spinal cord. Investigator chooses
spinal cord region, procedures of tissue preparation including
fixation, thickness of tissue sections, and quantity of slides. |
| STS-500 |
Floating
spinal cord sections |
Frozen
sections of 20-60 microns thickness for cervical, thoracic,
lumber, or sacral regions of adult rat or mouse spinal cord
in coronal orientation. Investigator chooses spinal cord region,
procedures of tissue preparation including fixation, thickness
of tissue sections, and quantity of floating sections. . |
| STS-550 |
Embryo
tissue sections |
Paraffin
sections in sagittal or coronal orientation for whole rat or
mouse embryos at different stages of pregnancy (mouse, from
E9 to E18 and rat embryo from E11 to E20). Investigator chooses
age of embryos, procedures of tissue preparation including fixation,
thickness of tissue sections, and quantity of slides. |
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